91亚洲欧美-91亚洲欧美综合高清在线-91亚洲区国产区精品区-91亚洲视频在线-91亚洲视频在线观看-91亚洲综合

實(shí)驗(yàn)標(biāo)準(zhǔn)品圖：

40 ng/L，5號(hào)標(biāo)準(zhǔn)品，150μl的原倍標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液

20 ng/L，4號(hào)標(biāo)準(zhǔn)品，150μl的5號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液

10 ng/L，3號(hào)標(biāo)準(zhǔn)品，150μl的4號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液

5 ng/L，2號(hào)標(biāo)準(zhǔn)品，150μl的3號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液

2.5 ng/L，1號(hào)標(biāo)準(zhǔn)品，150μl的2號(hào)標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液

大鼠尿激酶型纖溶酶原激活物受體(PLAUR/uPAR)elisa試劑盒48T/96T英文名稱：Rat urokinase type plasminogen activator receptor (PLAUR/uPAR) ELISA KitELISA試劑為我司主營(yíng)產(chǎn)品之一，產(chǎn)品皆嚴(yán)格按照相關(guān)標(biāo)準(zhǔn)進(jìn)行 ，并經(jīng)過(guò)了嚴(yán)格地反復(fù)地檢測(cè)，我們以嚴(yán)謹(jǐn)?shù)膽B(tài)度保證產(chǎn)品的質(zhì)量，從而保證您的實(shí)驗(yàn)順利進(jìn)行。產(chǎn)品供貨周期短，供貨及時(shí)，且我司還提供完整的售前和售后服務(wù)。別名:大鼠尿激酶型纖溶酶原激活物受體(PLAUR/uPAR)elisa檢測(cè)試劑盒產(chǎn)品規(guī)格:48T/96T。

產(chǎn)品名稱：大鼠尿激酶型纖溶酶原激活物受體(PLAUR/uPAR)elisa試劑盒48T/96T

英文名稱：Rat urokinase type plasminogen activator receptor (PLAUR/uPAR) ELISA Kit

規(guī)格 ：48T/96T

主要成分：酶標(biāo)板,試劑,標(biāo)準(zhǔn)品等。

試劑盒種屬：馬鈴薯、鹿、羊、雞、鴨、魚、人、大鼠、小鼠、豚鼠、倉(cāng)鼠、裸鼠、兔子、豬、犬、猴、馬、牛等動(dòng)植物。

檢測(cè)目的：用于測(cè)定血清，血漿及相關(guān)液體等樣本。產(chǎn)品僅用于科研例如適合檢測(cè)包括血清、血漿、尿液、胸腹水、灌洗液、腦脊液、細(xì)胞培養(yǎng)上清、組織勻漿等標(biāo)本..

操作流程如下：

（1）產(chǎn)品僅用于科研待測(cè)樣品孔中每孔加入待測(cè)樣品100μl，每種樣品設(shè)3個(gè)平行孔；設(shè)兩個(gè)陰性對(duì)照孔，每孔加未處理組的細(xì)胞裂解液100μl；另設(shè)一個(gè)空白對(duì)照孔，加入純細(xì)胞裂解液100μl?！?/span>

（2）酶標(biāo)板置4℃，包被過(guò)夜。

（3）洗板：吸干孔內(nèi)反應(yīng)液，用洗滌液過(guò)洗一遍（將洗滌液注滿板孔后，即甩去），之后將洗滌液注滿板孔，浸泡1-2分鐘，間歇搖動(dòng)。甩去孔內(nèi)液體后在吸水紙上拍干。重復(fù)洗滌3-4次。

（4）陰性對(duì)照孔每孔加入PBS 50μl，樣品孔及空白孔每孔加入1:500稀釋的兔抗人AIF抗體工作液50μl?！?/span>

（5）酶標(biāo)板置37℃培養(yǎng)箱的濕盒內(nèi)，孵育60min?！?/span>

（6）洗板，同（4）?！?/span>

（7）每孔加1:5000稀釋的HRP-標(biāo)記的山羊抗兔抗體工作液 100μl。　

（8）酶標(biāo)板置37℃培養(yǎng)箱的濕盒內(nèi)，孵育60min?！?/span>

（9）洗板，同（4）。　

（10）每孔加TMB顯色液 100μl，輕輕混勻10s，置37℃暗處反應(yīng)15-20min?！?/span>

（11）每孔加100μl 2mol/L H2SO4終止反應(yīng)?！?/span>

（12）分別測(cè)450nm 吸光值W1和630nm吸光值W2，zui終測(cè)得的OD值為兩者之差（W1-W2），以減少由容器上的劃痕或指印等造成的光干擾。

（13）數(shù)據(jù)處理：在得出標(biāo)本（S）和陰性對(duì)照（N）的 OD值后，計(jì)算S/N值。S/N≥2.1為陽(yáng)性判定標(biāo)準(zhǔn)。

注意事項(xiàng)：

1．從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用，酶標(biāo)包被板開封后如未用完，板條應(yīng)裝入密封袋中保存。

2． 濃洗滌液可能會(huì)有結(jié)晶析出，稀釋時(shí)可在水浴中加溫助溶，洗滌時(shí)不影響結(jié)果。

3． 各步加樣均應(yīng)使用加樣器，并經(jīng)常校對(duì)其準(zhǔn)確性，以避免試驗(yàn)誤差。一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4． 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線，做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔

*孔的OD值），請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定，計(jì)算時(shí)請(qǐng)*乘以總稀釋倍數(shù)（×n×5）。

5． 封板膜只限一次性使用，以避免交叉污染。

6． 底物請(qǐng)避光保存。

7． 嚴(yán)格按照說(shuō)明書的操作進(jìn)行，試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8． 所有樣品，洗滌液和各種廢棄物都應(yīng)按傳染物處理。

9． 本試劑不同批號(hào)組分不得混用。

以下是大鼠尿激酶型纖溶酶原激活物受體(PLAUR/uPAR)elisa試劑盒48T/96T的相關(guān)產(chǎn)品：

RecombinantHumanIL-1F1/IL-1aanti- ATP6V1B2 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanAmphiregulin/AREG/AREGB/CRDGF/SDGFanti- ATP6V1C1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanCCL28anti- ATP6V1C2 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanB7-2/CD86anti- ATP6V1D antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanPDCD1/Programmedcelldeathprotein1anti- ATP6V1E1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanSP-D/Pulmonarysurfactant-associatedproteinDanti- ATP6V1E2 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanCD160/NKreceptorBY5*nti- ATP6V1F antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanCD154/CD40L/TNFSF*nti- ATP6V1G1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantMouseGrowthHormone/Ghanti- ATP6V1G2 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantMouseCXCL15/Lungkineanti- ATP6V1G3 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanLAG3/CD223/Lymphocyteactivationgene3Proteinanti- ATP7B antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanMIF/Macrophagemigrationinhibitoryfactoranti- ATP7B antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanIL-17Aanti- ATP8A1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanIL-6Ra/IL6R/HABP2/Interleukin-6receptorsubunitalphaanti- ATP9A antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanPCSK9/ProproteinConvertase9(biotinylated)anti- ATPAF1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantHumanCD200/OX-2/MOX1/MOX2(Fcfusion)anti- ATPAF1 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified

RecombinantMouseGranulocyteColony-StimulatingFactor/G-CSF/CSF1(C-polyHis)anti- ATPAF2 antibodyPBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)Immunogen affinity purified
大鼠尿激酶型纖溶酶原激活物受體(PLAUR/uPAR)elisa試劑盒48T/96T禽腺病毒（7型）Human LPCAT3(Lysophospholipid acyltransferase 5) ELISA KitAlias LPCAT3(Lysophospholipidacyltransferase5)ELISAKitReactivity HumanRange 0.156-10ng/mlSensitivity 0.094ng/ml

禽腺病毒（12型）Human OXM(Oxyntomodulin) ELISA KitAlias OXM(Oxyntomodulin)ELISAKitReactivity HumanRange 0.313-20ng/mlSensitivity 0.188ng/ml

病毒Human SMAD2(Mothers against decapentaplegic homolog 2) ELISA KitAlias SMAD2|JV18|MADH2|MADR2|JV18-1|hMAD-2|hSMAD2|SMADfamilymember2(SMAD2|Smad2|hSMAD2)|JV18-1|Mad-relatedprotein2(hMAD-2)|ELISAKitReactivity HumanRange 0.313-20ng/mlSensitivity 0.188ng/ml

草魚病毒Human WNT3A(Protein Wnt-3a) ELISA KitAlias WNT3A|MGC119418|MGC119419|MGC119420|proteinWnt-3a|wingless-typeMMTVintegRationsitefamily|member3AELISAKitReactivity HumanRange 1.563-100ng/mlSensitivity 0.938ng/ml

多殺性巴氏桿菌Human Smad3/MADH3(Mothers Against Decapentaplegic Homolog 3) ELISA KitAlias Smad3|MADH3|JV15-2|SMADfamilymember3|hMAD-3|hSMAD3|MADhomolog3|madproteinhomolog|MAD|mothersagainstdecapentaplegichomolog3|MAD|mothersagainstdecapentaplegichomolog3(Drosophila)|mad3|Mad3|MADH3madhomologJV15-2|mothersagainstdecapentaplegichomolog3|MothersagainstDPPhomolog3|SMA-andMAD-relatedprotein3|SMADfamilymember3HSPC193|SMAD|mothersagainstDPPhomolog3|SMAD|mothersagainstDPPhomolog3(Drosophila)|Smad3ELISAKitReactivity HumanRange 0.625-40ng/mlSensitivity 0.375ng/ml

蜜蜂鏈球菌Human RPESP(RPE-spondin) ELISA KitAlias RPESP|C8orf84|FLJ40021ELISAKitReactivity HumanRange 0.156-10ng/mlSensitivity 0.094ng/ml

馬腺疫鏈球菌獸疫亞種Human SEMA3G(Semaphorin-3G) ELISA KitAlias SEMA3G|sem2|Semaphorinsem2ELISAKitReactivity HumanRange 0.313-20ng/mlSensitivity 0.188ng/ml